RESUMO Defeitos ósseos constituem um problema de saúde global. O sistema Rigenera permite a extração de microenxertos ricos em células-tronco mesenquimais (CTMs). Objetivou-se avaliar o processo de regeneração óssea por enxertos obtidos pelo sistema Rigenera em defeitos críticos na calvária de ratos. Foram utilizados 18 ratos Wistar, machos, pesando 285±29g, distribuídos em três grupos (n=6), sendo cada animal controle de si mesmo, denominados G15-Controle e G15-Tratado (15 dias); G30-Controle e G30-Tratado (30 dias) e G60-Controle e G60-Tratado (60 dias). Foram realizadas duas lesões de 5mm de diâmetro em cada antímero da calvária. Nos grupos tratados, foram utilizados microenxertos autólogos de cartilagem xifoide, obtidos pelo sistema Rigenera. O defeito contralateral serviu como controle em todos os animais. Os animais foram eutanasiados aos 15, 30 e 60 dias após a cirurgia, e as amostras foram processadas para a histoquímica. Nos grupos controle, não foram observados sinais de regeneração óssea, enquanto nos grupos tratamento foram verificadas áreas de formação óssea e tecido mesenquimal ativado. O sistema Rigenera foi eficiente na obtenção de microenxertos autólogos, para terapia celular em defeito crítico de calvária de ratos. Com o aprimoramento do protocolo, o sistema Rigenera poderá ser amplamente utilizado no tratamento de lesões ósseas.

ABSTRACT Bone defects are a global health problem. The Rigenera system allows the extraction of micro grafts rich in mesenchymal stem cells (MSCs). The objective of this study was to evaluate the bone regeneration process by grafts obtained by the Rigenera system in defects in the rats calvarian. Eighteen male Wistar rats were used, weighing 285 ± 29g, distributed in three groups (n = 6), where each animal was treatment and control, called G15-Control and G15-Treated (15 days); G30-Control and G30-Treated (30 days) and G60-Control and G60-Treated (60 days). Two 5mm diameter lesions were performed on each calvaria side. In the treated groups, autologous micrograft from xiphoid cartilage, obtained by the Rigenera system, were used. The other defect served as a control in all animals. The animals were euthanized at 15, 30 and 60 days after the surgery and the samples were processed for histochemistry. In the control groups, no signs of bone regeneration were observed, while in the treatment groups, areas of bone formation and activated mesenchymal tissue were verified. The Rigenera system was efficient in obtaining autologous micrograft for cell therapy in a critical calvaria defect in rats. Rigenera system can be widely used in the treatment of bone injuries.

The authors studied the Tei index calculated thorough the two-dimensional M-mode guided echocardiography and determined the normal value for the dog. They also compared the results obtained by this technique with normal Tei index value measured by flow wave Doppler technique. For this purpose 47 cardiologically healthy dogs were used and the obtained value for the M-mode technique was 0,4710 ± 0,178 while for the flow wave Doppler technique was 0,4327 ± 0,1842. The statistical processing through Student t technique demonstrated that the differences do not have statistical significance.

Los autores estudiaron el índice de Tei calculado por medio de ecocardiografía bidimensional en modo-M guiado y determinaron el valor normal para el perro. Asimismo compararon los resultados obtenidos por ésta técnica con el valor normal del índice de Tei medido por Doppler pulsado de flujo. A tal efecto se utilizaron 47 perros cardiológicamente sanos y el valor obtenido para la técnica en modo-M fue de 0,4710 ± 0,1778 mientras que para la técnica por Doppler de flujo fue de 0,4327 ± 0,1842. El procesamiento estadístico a través de la técnica t de Student demostró que las diferencias encontradas carecen de significación estadística.

The authors studied the response in dogs with varying degrees of congestive heart failure due to myxomatous valve disease the simultaneous administration of three drugs: spironolactone, benazepril and magnesium gluconate. The studied patients (25) hadn’t received medication at the time of the first evaluation and that association was administered at dose of 2 mg / kg; 0.25 mg / kg and 7.5 mg / kg respectively every 24 hours for a period of four months. The data obtained were processed statistically by performing test for normality ( Shapiro-Wilks test and Wilcoxon) and inference based on two-sample (“t” for paired samples) The results showed an overall clinical improvement, verified by history and clinical evaluation, improvement in ECG parameters and significant differences in serum urea and creatinine, in the regurgitate volume and systolic and diastolic blood pressure. No changes in two-dimensional echocardiographic parameters studied were observed

Los autores estudiaron la respuesta de perros con distintos grados de insuficiencia cardiaca congestiva motivada por mixomatosis de la válvula mitral a la administración simultánea de tres drogas: espironolactona, benazepril y gluconato de magnesio. Los pacientes estudiados (25) no habían recibido ninguna medicación a la fecha del inicio del estudio y la mencionada asociación se administró en dosis de 2 mg/kg; 0,25mg/kg y 7,5mg/kg respectivamente, cada 24 horas, por un período de cuatro meses. Los datos obtenidos fueron procesados estadísticamente realizando prueba de normalidad Shapiro- Wilks, test de Wilcoxon e inferencia basada en dos muestras (“t” para muestras apareadas). Los resultados obtenidos mostraron una mejoría clínica general, verificada por anamnesis y evaluación clínica, una mejoría en los parámetros electrocardiográficos y diferencias significativas en los valores de urea y creatinina, en el volumen regurgitante y en la tensión arterial sistólica y diastólica. No se observaron modificaciones de los parámetros ecocardiográficos bidimensionales estudiados.